試題詳解

70. What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with a recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes (endonucleases). III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA.
(A) III, II, IV, V, I
(B) IV, V, I, II, III
(C) II, III, V, IV, I
(D) III, IV, V, I, II
(E) III, I, IV, V, II