(a) What is "aptamer" in thiM mRNA? (2 points)

(a) No signal or weak signal (2 points)

(b) Non-specific bands (2 points)

(b) How does the change of thiM mRNA secondary structure affect translation initiation? (2 points)

43. What is a protospacer adjacent motif (PAM) sequence? Why is it important in designing a CRISPR/Cas experiment?

44. The "UK variant"--lineage B.1.1.7 of SARS-CoV-2 has raised widespread concern these days. In the UK variant, several nuclcotide mutations were found on the receptor-binding domain (RBD) of viral spike protein, but only one amino acid change N501Y was characterized. Please explain why other RBD nucleotide mutations are silent?

45. All eukaryotes have three different RNA polymerases. Match the name of each RNA polymerases in Column I to its correct description in Column II. Options from Column I can be used more than once. Column I (1) RNA polymerasc I (2) RNA polymerase II (3) RNA polymerase III Column II A. Transcribing the genes for the two large rRNA molecules. B. Transcribing most eukaryotic genes that encode proteins. C. Transc cribing the genes for 5S rRNA and other small RNA molecules. D. Transcribing the genes for tRNA.

45. Please at least list two enzymes that are involved in RNA editing.

44. Please describe the positive and negative regulation of lac operon.

43. Please explain the Wobble concept.

42. Please describe the molecular process (including all the molecules involved) of homologousrecombination in E. coli.

114年 - [無官方正解]114 國立清華大學_碩士班入學考試試題_生命科學暨醫學院甲組(生物與醫學科學組)：分子生物學#132699

113年 - [無官方正解]113 國立中正大學_碩士班招生考試試題_生物醫學科學系：分子生物學#136961

113年 - 113 國立成功大學_碩士班招生考試_藥理學研究所 :分子生物學#126004

113年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(B)#118906

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(C)#120020

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(B)#120007

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(D)#118608

112年 - 112 國立臺灣大學_碩士班招生考試：分子生物學(A)#118594

112年 - 112 國立清華大學_碩士班考試入學試題_生命科學暨醫學院甲組(生物與醫學科學組):分子生物學#118497

112年 - 112 國立中正大學_碩士班招生考試_生物醫學科學系分子生物:分子生物學#118496