2. Which two amino acids are completely positively charged at physiological pH? (8 points)

10. A 12-kb DNA fragment was digested with restriction enzymes HindIII and Pstlunder the following conditions:(1) Digestion with HindIII yielded fragments of 3 kb, 4 kb, and 5 kb.(2) Digestion with PstI yielded fragments of 2 kb, 5 kb, and 5 kb.(3) Concomitant digestion with both HindIII and PstI yielded fragments of 1 kb, 2kb, 2 kb, 3 kb, and 4 kb.Using this data, please construct a possible restriction map of the original 12-kb fragment. (8 points)

2. Which two amino acids are completely positively charged at physiological pH? (8 points)

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