試題詳解

2.The DNA polymerases are the workhorses for replication of genomic information in living cells. Which of the following descriptions about their biotechnological applications is not correct?
(A) During polymerase chain reaction (PCR), DNA polymerases often extend misprimed targets and primer-dimers, which are common sources of nonspecific amplification.
(B) During PCR, hot-start DNA polymerases reduce nonspecific amplification, increase yields, and allow convenient room temperature setup for high-throughput applications.
(C) Although archaeal DNA polymerases are extremely heat-stable, they may bc slow in synthesizing DNA due to lower processivity.
(D) High-fidelity DNA polymerases are enzymes with strong proofreading activity based on their 3'→5' exonuclease activity.
(E) DNA polymerases can be engineered with high specificity by introducing a strong DNA-binding domain of another protein without compromising polymerase activity.