25. Which of the following enzymes is used to search for CpG islands?
(A) HpaII
(B) EcoRI
(C) HindIII
(D) PvuI
(E) BamHI

26. A disadvantage of using a prokaryotic expression system for eukaryotic proteins is that the proteins are (A) Highly phosphorylated after translation (B) Highly soluble (C) Heavily glycosylated (D) Improperly folded (E) Over-expressed

27. The Shine-Dalgarno sequence can be found in (A) tRNA (B) 5S rRNA (C) 16S rRNA (D) 30S ribosome (E) mRNA

28. Which of the following is ideal for screening a protein expression library? (A) 3' RACE (B) 5' RACE (C) Labeled antibodies (D) RT-PCR (E) Real-Time PCR

29. In the construction of an expression vector, which of the following would you include in order to stimulate a high level of RNA synthesis? (A) P3 (B) T7 phage promoter (C) (D) His region (E) GFP

30. Which of the following cloning vectors would you use to clone an insert of size 500 kb? (A) Plasmid vector (B) Shuttle vector (C) Cosmid vector (D) Phage λ vector (E) Yeast artificial chromosome

31. Which of the following statements concerning the constitutive heterochromatin is     true    ? (A) Gene dense region (B) Centromeric location (C) High Cot value (D) Early replicating (E) None of above

32. Meiotic recombination is initiated at (A) Late interphase (B) Early to mid prophase (C) Mid to late prophase (D) The appearance of chiasmata (E)

33. A temperature sensitive mutant refers to (A) A mutation of one of anti-freeze genes (B) A mutation of one of the heat-shock genes (C) A specific type of gene mutation resulting in instability of gene product on non-permissive temperature (D) A mutation of gene resulting in unstable genome at non-permissive temperature (E) All of above

34. Walker and colleagues tested three bacteria for their ability to revert (from his to ) after UV irradiation. Results of their experiment are shown below. Which statement is     incorrect     regarding the function of UmuC? (A) umuC mutation decreases the reversion rate (B) Overexpression of UmuC increases the reversion rate (C) This result indicates that umuC is essential for faithful repair of UV-induced DNA damage. (D) Bacterium devoid of umuC activity was essentially unmutable. (E) None of above

35. Regarding DNA repair, which of the following statements is     incorrect    ? (A) DNA photolyase in E. coli is involved in breaking pyrimidine dimers formed by UV radiation. (B) Patients with Xenoderma pigmentosum are extremely sensitive to ultra violet irradiation due to defects in base excision repair. (C) Microsatellite instability is frequently found in patients with a defective mismatch repair. (D) Double-strand breaks in eukaryotes are repaired by homologous recombination and non-homologous end joining. (E) mismatch repair system of E. coli utilizes the DNA methylation to differentiate template and newly synthesized DNA strand.

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