試題詳解

30. Match each feature of the plasmid pBR322 (at left) with one appropriate description presented (at right) (see illustration of pBR322 below). Descriptions may be used more than once. (5%) 

_____ ampR sequence

 _____ori sequence

_____tetR

_____BamHI sequence 

_____Pstl sequence 

(A) permits selection of bacteria containing the plasmid
(B) a sequence required for packaging recombinant plasmids into bacteriophage
(C) origin of replicatio
(D) cleavage of the plasmid here does not affect antibiotic sequence resistance genes
(E)insertion of foreign DNA here permits identication of bacteria containing recombinant plasmids