34. Which type of RNA participates in nuclear export of mRNA?
(A) snRNA
(B) miRNA
(C)tRNA
(D) hnRNA

30. Which of the following is most likely to lead to a loss of gene function? (A) A sequence change in the 3' untranslated region. (B) A change from a TAA codon to a TAG codon in the coding region. (C) A change from T to C in the promoter region. (D) A frameshift mutation in the coding region.

31. A cDNA library differs from a genomic library in that (A) the cDNA was constructed from introns only. (B) genomic libraries are only stored in bacterial cell (C) cDNA libraries are more stable. (D)cDNA libraries only contain information from genes that have been transc

32. Protein sorting of anterograde cargo to different destinations within the Golgi complex occurs in the (A) cis-Golgi. (B) medial-Golgi. (C) trans-Golgi. (D) trans-Golgi network.

33. The only cells that can correctly attach sugars to proteins to form glycoprotein products are (A) yeast cells. (B) E. coli cells. (C) mammalian cells. (D) algal cells.

35. Synthesis of pre-rRNA occurs in the (A) cytoplasm. (B) endoplasmic reticulum. (C) extranucleolar area of the nucleus. (D) nucleolus.

36. Steroid hormones activate transcription by (A) binding to a nuclear receptor. (B) phosphorylating a protein kinase. (C) binding to specific cell-surface receptors. (D) inhibiting a histone deacetylase.

37. Transcriptionally inactive genes (A) are not located within nucleosomes. (B) often are methylated. (C) are not resistant to DNase I. (D) are always located within euchromatin.

38. What is the function of TFIIH in the transcription initiation complex? (A) binding to the TATA box (B) unwinding the DNA duplex (C) catalyzing the synthesis of RNA (D) all of the above

39. Protein X contains 7 transmembrane domains, with a short N-terminus and a long C-terminus. Following protein synthesis, the N-terminus of the protein faces the lumen (the inside) of endoplasmic reticulum (ER). After protein X is transported to the cell surface via exocytosis, you would expect the C-terminus of the protein X to be (A) extracellular. (B) cytoplasmic. (C) in the lumen ofER. (D) in the lipid bilayer of the plasma membrane.

40. The first step in the secretory pathway that should be inhibited by a dominant-negative mutant of SNARE proteins is (A) rouigh endoplasmic reticulum to Golgi transport. (B) intra Golgi transport. (C) trans Golgi network (TGN) transport to the plasma membrane. (D) TGN transport to the late endosome.