86. When oxygen is bound to myoglobin, the amino acid ________ is complexed to the iron ion of the heme group while ________ forms a hydrogen bond to the oxygen.
(A) cysteine; serine
(B) cysteine; histidine
(C) serine; cysteine
(D) histidine; histidine
(E) histidine; cysteine

82. Which of these statements about enzyme-catalyzed reactions is false? (A) The Vmax of a reaction can be attained at saturated concentration of substrate, even in the presence of a competitive inhibitor. (B) At saturating levels of substrate, the rate of an enzyme-catalyzed reaction is proportional to the enzyme concentration. (C) The rate of a reaction decreases steadily with time as substrate is depleted. (D) The Michaelis-Menten constant Km equals the [S] at which v = 1/2 Vmax. (E) The activation energy for the catalyzed reaction is the same as for the uncatalyzed reaction, but the equilibrium constant is more favorable in the enzyme-catalyzed reaction.

83. Which of the polypeptide is most likely to form a collagen structure? (A) IQEVERDIQEVERDIQEVERD (B) SRAGNRKIVLETW (C) TEDNFPAGKSILF (D) GAPGSPGPSGAP (E) NKASVEMAIRNGS

84. Which of the following is false regrading 2,3-bisphosphoglycerate (BPG)? (A) It binds at a distance from the heme groups of hemoglobin. (B) It binds with lower affinity to fetal hemoglobin than to adult hemoglobin. (C) It increases the affinity of hemoglobin for oxygen. (D) It is an allosteric modulator of hemoglobin. (E) It is normally found associated with the hemoglobin extracted from red blood cells.

85. Proline residues are infrequently found in ________due to their ________. (A) helices; decreased ability to serve as hydrogen-bond donors (B) helices; large positive charge that disrupts the repeating structure (C) sheets; decreased ability to serve as hydrogen-bond donors (D) sheets; large positive charge that disrupts the repeating structure (E) turns; decreased flexibility as an amino acid

87. Which of the following is associated for determining the dihedral angle (phi) of the peptide bonds? (A) CO-NH-Cα-CO (B) NH-Cα-CO-NH (C) Cα-CO-NH-Cα (D) HN-Cα-CO-NH (E) Hα-CO-NH-Hα

88. The PCR reaction mixture does not include ________. (A) all four deoxynucleoside triphosphates (B) DNA containing the sequence to be amplified (C) heat-stable DNA polymerase (D) DNA ligase (E) oligonucleotide primer(s)

89. Which of the following nuclear-encoded protein does not require signal peptide? (A) Secreted protein (B) Mitochondrial protein (C) Cytosolic protein (D) Plasma membrane protein (E) All of the above require signal peptide

90. Which of the following techniques is the best to quantify protein-protein interactions? (A) Co-immunoprecipitation (B) Affinity chromatography (C) Chemical cross-linking (D) Surface plasmon resonance (E) The two-hybrid system

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