申論題

3. Below are shown schematics of a 1,500bp PCR product and the pUC18 plasmid, with important restriction sites and their position in parentheses. You clone the PCR product between BamHI and EcoRI sites of pUC18.

For each lane of the agarose gel below, show the bands that will result from digesting your plasmid (pUC18 with your PCR product inserted between BamHI and EcoRI sites) with the indicated restriction enzymes. On the left and right sides of the gel are DNA ladders to serve as comparison. As shown above, the insert PCR product is 1,500bp, and pUC18 plasmid is 2,500bp.