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試卷名稱：111年 - 111 國立清華大學_碩士班考試入學試題_生命科學院 甲組、丁組：分子生物學#125321
年份：111年
科目：研究所、轉學考(插大)◆分子生物學
22. Recombinant spike protein of SARS-CoV-2 is currently used for COVID-19 vaccination. Which of the following approach is NOT applied for the recombinant protein production from eukaryotic cells?
(A) Identify coding sequence of target protein from NCBI, gene bank or GIASID database
(B) Generate an expression construct with the target gene driven by a T7 promoter
(C) Generate an expression construct with the target gene driven by a CMV promoter
(D) Incorporate the target gene with an extra tag (such as his, Fc, myc, flag etc) for purification
(E) Transfection the expression construct into a cell line such as CHO cells
