13.(題組)有一組科學家建構了以下的轉殖DNA,並將轉染細胞中。試回答列問題: [駐:mcheery為紅色螢光蛋白質,GFP為綠色螢光蛋白質,NLS:nuclear localization signalRES: internai ribosome binding site] (1)此DNA在細胞中將會做出幾種蛋白质?(2分) (2)推測此細胞在螢光顯微鏡下的樣態(2分) (3)若對此細胞施予elFAA抑制劑Allolaurintero,如何影響其螢光表現?(2分)

1.以下酵素或蛋白質,請簡述(1)存在於那一种生物體(大腸桿菌或人類),並參與在DNA structure, replication,epair,或recombination 系統(2)有什麼功能及作用機制(可參考下列適當項目填入:nucleosome structure,proofreading, mitochondrial DNA replication, processive DNA polymerase, non-processive DNA polymerase, base excision repair, mismatch repair, nucleotide excision repair, double-strand break repair, translesion synthesis, DNA helicase, topoisomerase, helicase, single strand binding protein, non-homologous end joining, homologous recombination, strand invasion, strand migration, Hollyday junction recognition, Hollyday junction resolution, transposition) : (a) DNA polymerasey(3分) (b) DNA polymeraseV(3分) (C)Mcm2-7 complex(3分) (d)MSH2/MSH6(3分) (e) Histones HI, H2A, H2B,H3,H4(3分) (f) UvrA,B,C(3分) (g) RUVAB,RUVC(3分) (h)RecA,RecBCD(4分) 問答題

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