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112年 - 國立臺灣大學_碩士班招生考試_生物化學暨分子生物學研究所:生化學#113391
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題組內容
15. Please explain the function of following post-translational modifications, and explain which amino-acid residues have these modifications. (8%)
3) Methylation.
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6) Ca2+ is an important second messenger in many signaling pathways. Please describe how the increase in intracellular Ca2+ concentration may promote the maturation and release of Insulin in beta cells. (4%)
#484608
5. In which direction along the template must the RNA polymerase in Figure below be moving to have generated the supercoiled structures that are shown? Would you expect supercoils to be generated if the RNA polymerase were free to rotate about the axis of the DNA as it progressed along the template? (4%)
#484609
6. Why is the drug azidothymidine (AZT) block the growth of the AIDS virus HIV. (2%)
#484610
1) How does this phage replicate its DNA?
#484611
2) Do you think any progeny phage will be 14C-labeled?
#484612
1 ) A telomere allows a chromosome to be replicated precisely so that no nucleotides are lost from the end of the chromosome, thereby solving the end-replication problem.
#484613
2 ) Nuclease-hypersensitive sites in chromatin are located in the linker DNA between nucleosomes.
#484614
3) If a DNA-binding protein forms a symmetric dimer, it is likely that the recognition site in the DNA is symmetric as well.
#484615
4) In general, the DNA replicating enzyme in E. coli is DNA polymerase I.
#484616
5) Loss of the 3' to 5' exonuclease activity of DNA polymerase in E. coli should slow the rate of DNA synthesis but not affect its fidelity.
#484617
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