5. Why is it important to regulate gene expression? Please provide an example of what would happen if gene expression is dysregulated.(5分)

1.以下酵素或蛋白質,請簡述(1)存在於那一种生物體(大腸桿菌或人類),並參與在DNA structure, replication,epair,或recombination 系統(2)有什麼功能及作用機制(可參考下列適當項 目填入:nucleosome structure,proofreading, mitochondrial DNA replication, processive DNA polymerase, non-processive DNA polymerase, base excision repair, mismatch repair, nucleotide excision repair, double-strand break repair, translesion synthesis, DNA helicase, topoisomerase, helicase, single strand binding protein, non-homologous end joining, homologous recombination, strand invasion, strand migration, Hollyday junction recognition, Hollyday junction resolution, transposition) : (a) DNA polymerasey(3分) (b) DNA polymeraseV(3分) (C)Mcm2-7 complex(3分) (d)MSH2/MSH6(3分) (e) Histones HI, H2A, H2B,H3,H4(3分) (f) UvrA,B,C(3分) (g) RUVAB,RUVC(3分) (h)RecA,RecBCD(4分) 問答題

2. What are different types of RNA found in the cell? (5分)

3. What is an operon in prokaryotes? Please provide an example of how operon works. (5 分)

4. Please describe what experimental approach would you choose if you would like to deplete a gene in eukaryotic cells and how to do it. (5分)

6. If you would like to study whether the expression of X gene is regulated by a specific transcriptional regulator (Y protein), what experiments would you perform? (5分)

10.何謂 suppressor RA?(4分)

11.簡述在細胞中如何籍由eiF2來調節整體轉譯效能・(5分)

12.試述RNApolymerase的CTD(C-terminaldoman)在調節轉錄活性的功能,(4分)

13.(題組)有一組科學家建構了以下的轉殖DNA,並將轉染細胞中。試回答列問題: [駐:mcheery為紅色螢光蛋白質,GFP為綠色螢光蛋白質,NLS:nuclear localization signalRES: internai ribosome binding site] (1)此DNA在細胞中將會做出幾種蛋白质?(2分) (2)推測此細胞在螢光顯微鏡下的樣態(2分) (3)若對此細胞施予elFAA抑制劑Allolaurintero,如何影響其螢光表現?(2分)

14. 假設你在研究過程中發現一個重要的A基因突可能與癌細胞轉移有高度相關性,有機可 做為臨床病人預後,在經過文献查,你大膽地擬定了一個假說如下,希望老師可以支持你進 行研究。 「A基因是一個位在細胞膜表面的酪胺酸激海受體(RTK,Receptor Tyrosine Kinase),他的突變 會造成A基因轉部出的蛋白质磷酸化程度提高,進而和B基因轉出的蛋白質交互作用,最後 让轉因子C進到细胞核中,表現與胞型態有關的基因,促進轉移,造成癌症病人預後差而 死亡。1 請問要證明上面的假設,有那些實驗需要進行請逐步設计實验方法及對要證明與回答的問 題。(10分)