47. Which type of columns are affected by the ionic charge, shape, substrate-binding strength of a protein, respectively? (A) Gel filtration, Affinity chromatography, Cation or anion exchange. (B) Affinity chromatography, Gel filtration, Cation or anion exchange. (C) Cation or anion exchange, Gel filtration, Affinity chromatography. (D) Gel filtration, Cation or anion exchange, Affinity chromatography. (E) Cation or anion exchange, Affinity chromatography, Gel filtration.